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You are responsible
for creating your own answers. I have given you the information you need in
previous lectures (from what I wrote on the board) for you to answer the
questions. Also, some of these concepts should have been discussed in the Bio
111 and Bio 112 courses you completed. It will be up to you to figure this
out. I will be happy to clarify what I
am asking you, but I will not help in answering the questions.
The purpose of this assignment is to begin to have you think
differently. Meaning this is not an assignment that will have you regurgitate
information. I want you to apply what you have learned in your previous
classes, and what I have discussed in lecture.
1.
Based on what you have learned from the cell
cycle, how many copies (give an estimate; you can state 1, more than 1, or
many) of DNA found in these organelles from C.
elegans and C. reinhardtii would
there be? Justify your answer using your knowledge of the cell cycle. (0.75 points)
2.
What would happen if organelle replication did
not occur for C. reinhardtii? When
answering this question think about the purpose/function of each organelle, and
include this in your answer. Note: I am not looking for elaborate answers. This
is a simple question. (1.5 points)
3.
C.
reinhardtii has a nuclear gene, tba1,
which encodes a protein needed for the translation of psbA mRNA in the chloroplast. The psbA mRNA translated results in the production of the D1 protein
(one of the core proteins of photosystem II). Scenarios listed in a-c below.
Below is a picture of a quadrant streak of C.
reinhardtii cells that you will label, as to which belongs to the
corresponding sample (sample 1, sample 2, sample 3). Also include an
explanation on your reasoning to why you selected the quadrant with growth or
no growth. Include in your explanation on a reason photosystem II is important
(I need a simple answer). (5.5 points)
a.
Sample 1- the psbA gene is on chromosome 2. During S phase, a spontaneous
mutation (change in the nucleotide sequence of a gene) occurs in one copy of
the psbA gene.
b.
Sample 2- the psbA gene is on chromosome 2. During S phase, a spontaneous
mutation (change in the nucleotide sequence of a gene) occurs in both copies of
the psbA gene.
c.
Sample 3- the psbA gene is on chromosome 2. During S phase, no mutations occur in
either copy of the psbA gene.
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