Microscopy for Microbiology - Use and Function

Lab Report Assistant

This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.

Exercise 1: Getting to Know your Compound Microscope

Data Table 1. Microscope Components.

Letter	Component Name	Component Function
A
B
C
D
E
F
G
H
I
J
K
L
M

Data Table 2. Total Magnification.

Lens	Ocular Magnification	Objective Magnification	Total Magnification
Scanning
Low Power
High Power
Oil Immersion

Data Table 3. Field of View.

Lens	Total Magnification	Field of View (mm)	Field of View (µM)
Scanning
Low Power
High Power
Oil Immersion

Data Table 4. Letter e Viewing Results.

Lens	Photograph	Observations
Scanning
Low
High
Oil Immersion

Questions

A.         Describe the details in the slides "Letter e" that become visible as the power changed from scanning power, to low power, to high power.

B.         Why is it important to calculate the diameter of the field when first using the microscope?

Exercise 2: Viewing Prepared Microbe Slides

Data Table 5. Prepared Slide Viewing Results.

Slide	Photograph	Total Magnification
Amoeba
Penicillium
Yeast
Spirillium
Bacillus
Coccus

Questions

A.         Using the field of view calculated in Exercise 1 for the high power lens, approximately how far across are each of the cells in the Bacteria Coccus Form slide in Data Table 5? Show your calculations.

B.         Detail techniques you found helpful for focusing on the various slides in this exercise.

Exercise 3: Preparing Wet-Mount Slides

Data Table 6. Wet-Mount Viewing Results.

Slide	Photograph	Total Magnification
Cheek Cell Smear
Dental Tatar Smear

Questions

A.      Describe the similarities and differences between the cheek cell wet mount and dental plaque wet mount.

B.      How did the process of preparing wet-mount slides become easier as you prepared the second wet-mount slide of this exercise?

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